|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues (C-E(69) L L D E D Q K V R P N E E(82) of human BDNF precursor.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1-5 µg/ml|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with human, mouse and rat proBDNF and is also expected to recognise other mammalian proBDNF. No cross-reactivity with other proneurotrophins was detected in Western blot.
Reconstitute with 20 ul of distilled water. After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.