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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • uNOS Antibodies

          Invitrogen

          uNOS Polyclonal Antibody

          7 Published Figures
          35 References
          View all (3) uNOS antibodies

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          Datasheet
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          Cite uNOS Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (7)
          uNOS Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          uNOS Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          uNOS Antibody (PA1-039) in IHC

          Immunolocalization staining of NOS in drosophila optic nerve using Product # PA1-039. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Immunohistochemistry (IHC)
          uNOS Antibody in Western Blot (WB)

          Product Details

          PA1-039

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 8 publications 8 publications

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100-1:200
          View 10 publications 10 publications
          Product Specifications

          Species Reactivity

          Arthropod, Bovine, Fruit fly, Human, Mouse, Pig, Rat

          Published species

          Arthropod, Cnidarian, Crab, Fish, Fruit fly, Human, Insect, Mollusc, Mouse, Pig, Rabbit, Rat, Shark

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of murine iNOS and bNOS.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325776

          Product Specific Information

          PA1-039 detects mouse macrophage inducible nitric oxide synthase (iNOS); rat, bovine, Drosophila, and porcine brain nitric oxide synthase (bNOS); human, porcine and bovine epithelial nitric oxide synthase (eNOS).

          PA1-039 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse macrophage iNOS; an ~155 kDa protein representing rat, bovine and porcine bNOS; ~140 kDa protein representing porcine and bovine eNOS. Immunohistochemical staining of NOS in Drosophila optic tissue with PA1-039 results in staining of the optic nerve. In immunohistochemistry procedures PA1-039 also stains NOS in human endothelial cells, macrophages, and peripheral neural tract cells.

          The PA1-039 immunogen is a synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of mouse iNOS and bNOS. This sequence is highly conserved among the different NOS isoforms.

          Target Information

          NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: bNOS; cNOS; Constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; HEP-NOS; Hepatocyte NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, brain; Nitric oxide synthase, endothelial; Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric-oxide synthase; nNOS; NOS type I; NOS type II; NOS type III; NOS, type II; NOSII; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; Peptidyl-cysteine S-nitrosylase NOS2

          View more View less

          Gene Aliases: 2310005C01Rik; 2310065A03Rik; Bnos; cNOS; EC-NOS; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; Inosl; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS1; NOS2; NOS2A; NOS3; NOSIII

          View more View less

          UniProt ID: (Human) P29475, (Pig) P79290, (Human) P35228, (Bovine) Q27995, (Pig) Q28969, (Human) P29474, (Rat) P29476, (Mouse) Q9Z0J4, (Mouse) Q8R410, (Rat) Q06518, (Mouse) P70313, (Rat) Q62600

          View more View less

          Entrez Gene ID: (Pig) 100520934, (Human) 4842, (Pig) 396859, (Human) 4843, (Bovine) 282876, (Bovine) 287024, (Pig) 397557, (Human) 4846, (Rat) 24598, (Mouse) 18125, (Mouse) 18126, (Rat) 24599, (Mouse) 18127, (Rat) 24600

          View more View less

          Function(s)
          nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding metal ion binding receptor binding actin monomer binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding actin binding beta-catenin binding cAMP-dependent protein kinase regulator activity oxidoreductase activity protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase protein modifying enzyme
          Process(es)
          response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction nitric oxide mediated signal transduction myoblast fusion female pregnancy aging negative regulation of cell proliferation response to heat response to lead ion negative regulation of calcium ion transport into cytosol response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine neurotransmitter biosynthetic process exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen cell redox homeostasis response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine regulation of neurogenesis negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation negative regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction prostaglandin secretion interleukin-6 production interleukin-8 production regulation of cytokine production involved in inflammatory response positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa superoxide metabolic process circadian rhythm response to bacterium negative regulation of gene expression cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration regulation of insulin secretion defense response to Gram-negative bacterium positive regulation of killing of cells of other organism cellular response to lipopolysaccharide cellular response to interferon-gamma interleukin-6 secretion interleukin-8 secretion mitochondrion organization blood coagulation cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion positive regulation of blood vessel diameter angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin regulation of blood pressure negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development lipopolysaccharide-mediated signaling pathway response to fluid shear stress endothelial cell migration positive regulation of angiogenesis regulation of blood vessel size regulation of nitric-oxide synthase activity muscle contraction regulation of heart contraction response to organonitrogen compound response to organic cyclic compound response to nutrient levels negative regulation of peptidyl-serine phosphorylation cellular response to epinephrine stimulus inflammatory response regulation of protein kinase activity endothelial cell proliferation signal transduction G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger response to mechanical stimulus response to hormone glycoside metabolic process cGMP-mediated signaling response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing positive regulation of apoptotic process retinoic acid receptor signaling pathway cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state response to dexamethasone negative regulation of smooth muscle cell proliferation cellular response to transforming growth factor beta stimulus response to fructose response to metal ion response to cytokine cellular response to heat response to statin response to drug response to amino acid response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
          It has to be done as per old AB suggested Products section.
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