PA1-039 detects mouse macrophage inducible nitric oxide synthase (iNOS); rat, bovine, Drosophila, and porcine brain nitric oxide synthase (bNOS); human, porcine and bovine epithelial nitric oxide synthase (eNOS).
PA1-039 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse macrophage iNOS; an ~155 kDa protein representing rat, bovine and porcine bNOS; ~140 kDa protein representing porcine and bovine eNOS. Immunohistochemical staining of NOS in Drosophila optic tissue with PA1-039 results in staining of the optic nerve. In immunohistochemistry procedures PA1-039 also stains NOS in human endothelial cells, macrophages, and peripheral neural tract cells.
The PA1-039 immunogen is a synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of mouse iNOS and bNOS. This sequence is highly conserved among the different NOS isoforms.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BNOS; cNOS; Constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; HEP-NOS; Hepatocyte NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, brain; Nitric oxide synthase, endothelial; Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric-oxide synthase; nNOS; NOS type I; NOS type II; NOS type III; NOS, type II; NOSII; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: 2310005C01Rik; 2310065A03Rik; Bnos; cNOS; EC-NOS; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; Inosl; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS1; NOS2; NOS2A; NOS3; NOSIII
Entrez Gene ID: (Pig) 100520934, (Human) 4842, (Pig) 396859, (Human) 4843, (Bovine) 282876, (Bovine) 287024, (Pig) 397557, (Human) 4846, (Rat) 24598, (Mouse) 18125, (Mouse) 18126, (Rat) 24599, (Mouse) 18127, (Rat) 24600