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uPA Polyclonal Antibody

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uPA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 17968-1-AP (uPA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

uPA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

uPA Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

uPA Antibody in Immunoprecipitation (IP) — IP result of anti-uPA (IP:17968-1-AP, 4ug; Detection:17968-1-AP 1:1000) with HeLa cells lysate 2800ug.

Product Details

17968-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	uPA Fusion Protein Ag11813 (82-431 aa encoded by BC013575) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: ASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA L (82-431 aa encoded by BC013575)

Target Information

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Ahmmed B,Kampo S,Khan M,Faqeer A,Kumar SP,Yulin L,Liu JW,Yan Q

Mon Jul 01 00:00:00 EDT 2019

Evaluation of the Inhibitory Effects of Genipin on the Fluoxetine-Induced Invasive and Metastatic Model in Human HepG2 Cells.

Molecules (Basel, Switzerland)

Tian YS,Chen KC,Zulkefli ND,Maner RS,Hsieh CL

Fri Dec 14 00:00:00 EST 2018

Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.

International journal of biological macromolecules

Sun D,Liu Y,Wang H,Deng F,Zhang Y,Zhao S,Ma X,Wu H,Sun G

Sun Apr 01 00:00:00 EDT 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 EDT 2018

α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression.

Oncogenesis

Chen H,Xu Z,Li X,Yang Y,Li B,Li Y,Xia K,Wang J,Li S,Wang M,Wu H

Tue Mar 13 00:00:00 EDT 2018

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Scientific reports

Liu Y,Wang S,Sun D,Liu Y,Liu Y,Wang Y,Liu C,Wu H,Lv Y,Ren Y,Guo X,Sun G,Ma X

Tue Jul 19 00:00:00 EDT 2016

Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches.

Oncology reports

Su B,Su J,He H,Wu Y,Xia H,Zeng X,Dai W,Ai X,Ling H,Jiang H,Su Q

Fri May 01 00:00:00 EDT 2015

Inhibition of plasmin protects against colitis in mice by suppressing matrix metalloproteinase 9-mediated cytokine release from myeloid cells.

Gastroenterology

Munakata S,Tashiro Y,Nishida C,Sato A,Komiyama H,Shimazu H,Dhahri D,Salama Y,Eiamboonsert S,Takeda K,Yagita H,Tsuda Y,Okada Y,Nakauchi H,Sakamoto K,Heissig B,Hattori K

Sun Mar 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: antagonist of uPA; HGNC:9052; plasminogen activator; plasminogen activator, urinary; U-plasminogen activator; uPA; UPAR; Urinary plasminogen activator, urokinase; URKR; Urokinase-type plasminogen activator; Urokinase-type plasminogen activator chain B; Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A

Gene Aliases: ATF; BDPLT5; PLAU; QPD; u-PA; UPA; UPAM; URK

UniProt ID: (Human) P00749, (Mouse) P06869

Entrez Gene ID: (Human) 5328, (Rat) 25619, (Mouse) 18792

Molecular Function: serine protease