Serum and Plasma miRNAs: a Guide to Sample Preparation
From sample to answer, learn about methods for optimal sample preparation in studies of miRNAs from serum and plasma
Circulating miRNAs in serum and plasma have shown potential in biomarker discovery and diagnostic screening. Serum and plasma are easier and less invasive to collect than traditional tissue biopsies. These "liquid biopsy" samples and their circulating miRNAs can provide a sensitive method to discover and monitor diseases.
Due to the potential for variability, several factors must be considered when studying miRNA from serum or plasma. These factors include:
- Sample type (serum or plasma), collection, handling, and storage
- RNA and miRNA extraction, quantity and quality
- Methods to analyze expression
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