Applied Biosystems™

TaqMan™ Advanced miRNA Human Serum/Plasma Card

Catalog number:  A34717

 Related applications:

Microarray Analysis

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Description

The Applied Biosystems TaqMan Advanced Serum/Plasma Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains one card for profiling of up to 188 unique miRNAs in two serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma Card include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 µL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v22
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details

Number of cards: 1
Number of unique miRNAs (not including exogenous controls): 188
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For more information on the availability and pricing for Advanced miRNA assays in pre-spotted 384 well plates, please email specialty_cards@thermofisher.com.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well microfluidic card
Research Category: miRNA
Detection Method: Primer-Probe Detection
Label or Dye: 2 Color
Product Line: TaqMan™
Product Size: 384 reactions
Species: Human
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

One 384-well array

The TaqMan miRNA card is shipped at ambient temperature. Upon arrival, store at 2–8°C and keep protected from light.

Documents

Manuals & protocols