High-Content Analysis (HCA)
High-content screening (HCS) or high-content analysis (HCA) describes a set of analytical methods using automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. Thermo Scientific CellInsight CX5, CellInsight CX7 and CX7 LZR, and ArrayScan high-content analysis products have directly impacted every segment of biological research into cellular and systems biology and drug discovery.
BPAE cells imaged using the Thermo Scientific CellInsight CX5 platform and stained with DAPI (blue), Invitrogen Alexa Fluor 568 phalloidin (red), goat anti–mouse IgG (H+L) secondary antibody, and Alexa Fluor 488 conjugate (green).
A549 cell image acquired using the CellInsight CX7 platform and stained with Invitrogen HCS CellMask Blue (blue), Alexa Fluor 488 Phalloidin (green), and Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).
HCS reagent selection guides
Drawing on decades of experience in fluorescence imaging, Invitrogen HCS products are developed using Thermo Scientific high-content platforms with special considerations for the high-throughput workflow and automated imaging:
- Cell and nuclear masks for automated demarcation
- Robust functional probes for cell health interrogation
- Flexible assay workflow for automated processing
- Broad fluorophore choice for easy multiplexing
- Validated on multiple imaging platforms
Select from the reagents optimized for use with your HCS platform:
Other cell structure and segmentation tools
Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Invitrogen Molecular Probes cell structure labels are widely used in microscope imaging and HCS.
Other cell health and cytotoxicity assays
We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.
Browse cell health and cytotoxicity assays by application:
Cell Analysis Support Center
Find tips, troubleshooting help, and resources for your cell analysis workflow.
For Research Use Only. Not for use in diagnostic procedures.