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Enable consistent T cell activation with CTS cell therapy reagents
Achieving reproducible and scalable T cell activation can be difficult due to donor variability. Manual isolation steps can influence performance. These factors can affect timing control and create variation in downstream work. Gibco CTS cell therapy reagents allow for more flexible activation approaches. The Gibco CTS Detachable Dynabeads Release Buffer offers active bead detachment to adjust activation timing, while the Gibco Soluble DynActivator CD3/CD28 Prototype*† supports bead-free activation that can occur independently of isolation steps. Both support T cell activation from development through manufacturing.
Benefits of CTS cell therapy reagents
CTS T cell reagents helps manage timing, bead removal, and activation duration, supporting consistent activation. These product formats fit within closed, automated environments, bridging activation steps to later stages of the workflow. These solutions can be used in autologous and allogeneic settings to support flexible process design.
Optimizing isolation and activation workflows
Isolation and activation steps establish the conditions that influence cell quality as they progress toward expansion. CTS cell therapy reagents are designed to support these transitions within closed or automated unit operations, enabling management of bead-based or bead-free activation with greater control.
| CTS Detachable Dynabeads Release Buffer** | Soluble DynActivator CD3/CD28 Prototype† |
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Support activation control and T cell phenotype preservation
The strength and duration of CD3/CD28 activation directly influence T cell phenotype, viability, and downstream function. Both activation formats offer ways to manage these parameters across diverse starting materials, supporting efforts to maintain desired T cell subsets as cells move into expansion.
| CTS Detachable Dynabeads Release Buffer** | Soluble DynActivator CD3/CD28 Activator Prototype† |
Controlled bead detachment allows users to adjust activation exposure within a closed workflow that helps preserve target phenotypes through early culture. |
This bead-free option offers controlled activation that can be adjusted through concentration and exposure time. Reagent removal via simple washing or medium exchange, such as on the CTS Rotea Counterflow Centrifugation System, supports flexibility in the manufacturing process. |
Enhance cell therapy workflows with connected solutions
Integrate Dynabeads cell separation technology with cell therapy products to support consistent handling from isolation through final processing.
Ordering information
Related resources
Frequently asked questions
CTS cell therapy reagents support the integral isolation and activation steps of T cell therapy workflows. They are available in both bead-based and bead-free formats, and can be paired with closed, automated systems, such as the CTS DynaCellect Magnetic Separation System for cell isolation and the CTS Rotea Counterflow Centrifugation System for washing and volume reduction.
CTS Detachable Dynabeads Release Buffer uses a biotin-based competitive release mechanism to actively detach CTS Detachable Dynabeads CD3/CD28 at your selected timepoint. Excess biotin in the buffer competes with the bead-cell interaction, allowing users to release beads rather than rely on passive dissociation. This approach supports modulation of CD3/CD28 activation strength and duration in both manual and automated workflows. It offers greater flexibility in timing and controlling activation conditions across cell therapy manufacturing steps.
Soluble DynActivator CD3/CD28 Prototype† is a soluble reagent that is removed through routine medium exchange or simple washing once the activation window is met. For teams working with closed or larger-scale formats, the CTS Rotea Centrifugation System can handle washing and buffer exchange in a controlled setup. These options make it simple to set defined activation times and move easily into downstream culture or engineering steps.
CTS Detachable Dynabeads Release Buffer should be stored at 2–8°C and kept protected from light. Soluble DynActivator CD3/CD28 Prototype† ships frozen and is stored at –15°C to –25°C until use. Each reagent for cell therapy should be handled using standard laboratory protective measures and mixed gently to maintain product integrity. Following these storage and handling guidelines helps keep the reagents ready for use.
Additional bioprocessing resources
Bioprocessing resources
Product Name |
Regulatory Statement |
CTS Detachable Dynabeads Release Buffer |
**For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals. |
CTS DynaCellect Magnetic Separation System |
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CTS Rotea Counterflow Centrifugation System |
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CTS Xenon Electroporation System |
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Soluble DynActivator CD3/CD28 Prototype |
†For Research Use Only. Not for use in diagnostic procedures. Product is a prototype and performance characteristics of this product have not been established. |
* Gibco Soluble DynActivator CD3/CD28 Prototype (“Prototype”) is not yet a commercially released product and is still under development. Any use of the Prototype is done at the sole risk and discretion of the customer. Additionally, the customer understands and agrees that the Prototype is provided “AS IS” and without warranty of any kind, express, implied, or statutory. All warranties are expressly disclaimed, including without limitation, those of merchantability or fitness for a particular purpose, and non-infringement of third-party intellectual property rights, whether arising from statute or in law or from a course of dealing or usage of trade. Thermo Fisher disclaims all responsibility or liability for customer’s use of the Prototype (or any portion thereof), including but not limited to any liability or responsibility under Thermo Fisher’s standard terms of sale, a negotiated master supply agreement, or a quality agreement. The Prototype is provided under Thermo Fisher’s standard terms and conditions located at thermofisher.com/us/en/home/global/terms-and-conditions.html except as specifically modified by these Prototype Terms. If you have questions, contact Thermo Fisher at thermofisher.com/support.