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10,000
Analyze 10,000 files in less than 5 minutes
 
2.5x
Up to 2.5x
faster analysis
 

Applied Biosystems Sanger Analysis Modules are innovative cloud-based secondary data analysis tools that bring together multiple data sets in one convenient place. This online solution makes it easier to view, store, and analyze Sanger sequencing data. The Sanger Analysis Modules are compatible with files from the Applied Biosystems 310, 3130 Series, 3730 Series, and 3500/3500xL Genetic Analyzers.

New! Microsatellite Analysis Software

Microsatellite Analysis Software (MSA) is a microsatellite genotyping software that allows you to analyze a mixture of DNA fragments, separated by size. This analysis provides a profile of the separation, precisely calculates the sizes of the fragments, and determines the microsatellite alleles present in the sample.

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Quality Check

For basic quality check. View .ab1 data and compare performance against thresholds

Free access on the cloud

Variant Analysis

For reference-based re-sequencing analyses

Free access on the cloud

NGS Confirmation

For examination of CE variants to confirm variants detected by NGS platforms

Free access
on the
cloud

Peak Scanner

For basic fragment quality check. View .fsa data, fragment sizes and identify peaks

Free access on the cloud

Microsatellite Analysis

For genotyping based on DNA fragment size and microsatellite alleles present in sample

Free access on the cloud

Ease of use with step-by-step interface yes yes yes yes yes
ease of use

The new intuitive interface provides step-by-step guidance in importing, analyzing and exporting data. Sanger data analysis is easier than ever before.

NGS confirmation     yes    
NGS confirmation

The module provides one central place to confirm your NGS variants. It takes CE sequencer–generated .ab1 files and NGS variant .vcf files, compares variants within the same view and generates a Venn diagram for reporting.

Variant reporting in absolute genomic coordinates   yes yes    
absolute genomic coordinates

The modules are the only Sanger sequencing software on the market that reports variants in absolute genomic coordinates and not relative to a reference. This eliminates the need to calculate absolute genomic coordinates from the relative location of a reference sequence.

.vcf output for downstream analysis   yes yes    
vcf output

The modules generate and export variants in the standard variant call format (.vcf), which is the standard file format in bioinformatics for storing gene sequence variations. This enables downstream analysis to search multiple databases using the Ion Reporter™ annotation workflow.

Automated database search   yes yes    
automated database search

The modules can automatically retrieve reference sequences from genomic databases, report variants in genomic coordinates and report genomic annotations for SNPs.

High processing power (10,000 files in less than 5 minutes)   yes yes yes yes
high processing power

High-output labs will no longer be slowed down by data analysis software. The module can process up to 10,000 samples in less than 5 minutes, which enables you to keep up with ever-growing demand.

Fragment sizing       yes yes

This software uses the size standard in each sample to create a standard curve. It then determines the relative size of each dye‐labeled fragment in the sample by comparing fragments with the standard curve for that specific sample.

Genotyping         yes

This analysis software assigns allele calls based on user‐defined makers (loci).

Featured videos

Microsatellite Analysis Software

Applied Biosystems Microsatellite Analaysis Software (MSA) is for genotyping based on DNA fragment size and microsatellite alleles present in sample.


Thermo Fisher Cloud module provides an automated workflow to confirm your NGS variants

If you work in a clinical research lab, you are likely familiar with the manual process of comparing data from your NGS platform with data from your Sanger sequencing system. It is a time-consuming process that varies with the quality of your data. Well, good news! NGS variant confirmation is made easy with the Applied Biosystems NGS Confirmation Software.

At ASHG 2015 Venkatesh Moktali led two live demo sessions at the Thermo Fisher Scientific booth, giving customers a glimpse of how this new software solution can streamline their NGS variant confirmation workflow. We spoke briefly with Venkatesh before his demo about the benefits of the software.

working on computer in the lab

Transform your core lab

Find out how you can bring your service lab to the next level with innovative
new features of the Sanger Analysis Modules.

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