For a free version of our Next-generation Confirmation Software, visit our Apps Page (opens a new window) on Thermo Fisher Cloud.

Analysis Module Next-Generation Confirmation (NGC) Software

Catalog number: A28221

Applied Biosystems™  Related applications: Sanger Sequencing

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Applied Biosystems® Analysis Module Next-Generation Confirmation (NGC) is CE Sanger sequencing software hosted on the Thermo Fisher Cloud environment. NGC can be accessed for free through the Apps Page. It confirms variants generated by:

• Detecting variants from CE sequence ab1 files
• Comparing NGS variants and CE variants
• Generating Venn diagrams for visual confirmation

Access NGC software for free >

With Next-Generation Confirmation software, you can examine variants from a CE electropherogram to confirm the variants detected by NGS platforms. The software takes CE sequencer-generated ab1 files and performs SNP detection and analysis, SNP discovery and validation, and sequence confirmation, all on the cloud. NGC software can automatically retrieve reference sequence from genomic databases, report variants in genomic coordinates, and report genomic annotations for SNPs. The software takes NGS variant vcf files and compares NGS variants and Sanger variants in the same alignment view and can also generate a Venn diagram, allowing the user to visually compare and confirm variants generated from NGS. In addition, the NGC software generates and can export variants in standard variant call format (vcf).

Note: Confirmation of heterozygous deletions and insertions will be supported in future software release.

Key software features:
• Variants generated from NGS platforms confirmed
• Variants generated from NGS and CE platforms displayed in the same view
• Simplified workflow requires less than a half hour to learn
• No operating system limitation (including iOS). Software accessed through web browser.
• No software maintenance needed from user
• Increased analysis capacity through power of the cloud
• Projects can be shared among registered users for collaboration
• Genomic coordinates reported for each variant and genomic annotations automatically retrieved
• Easy navigation to regions of interest in electropherograms
• Simple quality checking by color-coded trace summary and quick look-ups of markers (low QV, user-edited bases, mixed bases, etc.)
• Automatic alignment of multiple analyzed plots, forward or reverse complementary
• Variants exported in standard vcf format

For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 3700 Genetic Analyzer, 3500 Genetic Analyzer, 310 Genetic Analyzer, 3100 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, 3100-Avant™ Genetic Analyzer, 3130 Genetic Analyzer, 3730 DNA Analyzer
Software Type: Web Based Software
Product Size: 1 license


Manuals & protocols