Revolutionizing Gene Editing with CRISPR and Digital PCR – A Conversation with Dr. Dustin Rubinstein

In a recent Absolute Gene-ius podcast episode titled “CRISPR, dPCR, and pigs – a cut above the rest”, Dr. Dustin Rubinstein, Director of the Advanced Genome Editing Laboratory at the University of Wisconsin-Madison, shared his insights on how CRISPR and digital PCR (dPCR) are revolutionizing gene editing workflows. This Q&A format blog post captures the essence of his discussion, focusing on the applications of these technologies in cancer research and rare disease modeling.

Listen to the full episode on the Absolute Gene-ius webpage.

Q: How are genetically engineered pig models used in research?

Dr. Rubinstein: Genetically engineered pig models offer more clinically relevant insights compared to traditional small animal models. By using CRISPR to modify pig genomes, researchers can mimic human disease conditions more accurately. These models are invaluable for cancer and rare disease research, helping us understand the genetic mechanisms underlying these diseases and explore potential therapies.

Q: What is digital PCR (dPCR), and why is it important in gene editing workflows?

Dr. Rubinstein: Digital PCR (dPCR) provides absolute quantification and unparalleled precision in detecting and confirming gene edits. Unlike traditional qPCR and sequencing methods, dPCR eliminates ambiguity, making it particularly advantageous for copy number variation (CNV) analysis and gene editing confirmation. This precision is vital for applications such as cancer research, where the presence or absence of specific gene copies can significantly impact disease progression and treatment outcomes.

Q: How do CRISPR and dPCR complement each other in research workflows?

Dr. Rubinstein: The integration of CRISPR and dPCR in research workflows offers a powerful combination for academic researchers. By leveraging these technologies together, scientists can achieve precise genetic modifications and validate them with high accuracy. This synergy is transforming various aspects of molecular biology, from basic science to translational research. In my lab, we use CRISPR and dPCR to develop pig models for studying neurofibromatosis and cancer, ensuring that the modifications are accurate and reliable.

Q: Can you share some insights on the importance of mentorship in science?

Dr. Rubinstein: Mentorship has played a crucial role in my career and the development of my lab. For young researchers entering the field of gene editing, having experienced mentors can provide valuable guidance and support, helping them navigate the complexities of scientific discovery. My journey from founding a CRISPR core to leading an advanced genome editing laboratory underscores the collaborative nature of modern molecular biology. Working with diverse teams and sharing knowledge across labs is essential for driving innovation and achieving breakthroughs in genetic research.

Q: What are some of the latest advancements in CRISPR technology?

Dr. Rubinstein: CRISPR technology continues to evolve, with new variations such as base editing and prime editing emerging. These advanced techniques offer even greater precision and flexibility, allowing researchers to target specific genetic sequences without cutting the DNA. The potential applications of these technologies are vast, from curing genetic diseases to developing novel therapies. Staying updated with the latest advancements in CRISPR and dPCR is crucial for academic researchers.

Q: How can researchers keep up with the rapid pace of innovation in gene editing?

Dr. Rubinstein: Keeping up with the rapid pace of innovation in gene editing can feel like “sipping from a fire hose.” However, understanding the capabilities and limitations of these tools is essential for making informed decisions in research workflows. Regularly engaging with the scientific community through conferences, seminars, and podcasts can help researchers stay informed about the latest developments and best practices.

Conclusion

CRISPR and digital PCR are revolutionizing gene editing workflows, offering academic researchers powerful tools for exploring genetic modifications and developing disease models. The precision and efficiency of these technologies are transforming our understanding of complex genetic networks and paving the way for innovative therapies. By integrating CRISPR and dPCR, researchers can achieve accurate and reliable gene edits, advancing the field of molecular biology and contributing to the future of precision medicine.

Learn more about dPCR and Thermo Fisher Scientific’s dPCR portfolio at thermofisher.com/dPCR