Real-Time PCR Detection of Vaginal Microbiota

Traditional culture-based or microscopy-based methods for investigations into vaginal microbiota can lack sensitivity and specificity and can also be subjective, time-consuming and inaccurate.

Real-time PCR can detect slow-growing, difficult-to cultivate, or uncultivable microorganisms, and can be used when traditional microbiological techniques lead to ambiguous results. Using real-time PCR techniques along with microorganism-specific TaqMan Assays enables rapid and accurate detection and categorization of microorganisms involved in bacterial vaginosis, aerobic vaginitis and vaginal candidiasis.

We have developed a flexible, low-cost and high-throughput real-time PCR testing solution for vaginal microbiota profiling.* This solution includes a single protocol optimized for DNA isolation from different types of vaginal microbes, a set of validated TaqMan Assays covering 28 pathogenic and commensal microbes, plus four control assays, including human (RNase P) and bacterial (16S) targets.

Key Features:

The ability to detect the broadest range of both commensal and pathogenic microbes compared to other commercially available molecular tests
Qualified content, including positive controls, user guide and analytical testing
Higher specificity, accuracy and precision compared to traditional culture and microscopy methods
Flexible formats—choose from four assay formats, including single-tube assays and preloaded 384-well or OpenArray™ plates
Lowest cost per sample compared to other commercially available solutions

Find out more about our vaginal microbiota PCR solutions at thermofisher.com/vm

*For research use only. Not for use in diagnostic procedures.