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Expertise

GeneticsMolecular Virology

Education

B.S. in genetics at Cornell University; Ph.D. in molecular virology at University of Alabama at Birmingham

John Pfeifer, PhD

Sr. Real-Time PCR Field Applications Scientist, Thermo Fisher Scientific

John graduated by Cornell University with a B.S. in genetics, followed by a Ph.D. in molecular virology at the University of Alabama at Birmingham.   He was then a post-doctoral research fellow at Harvard University, and then a research associate at Baylor College of Medicine.  In 1991, he was hired by the Perkin Elmer Corporation to support PCR as a field applications scientist (FAS).

In 1994, John supported the first use of TaqMan probes for end-point applications.  With the introduction of a new type of PCR called real-time PCR in 1996, he was one of the first 4 FAS supporting the technology in the U.S.

Through multiple corporate acquisitions, today the real-time PCR product line is part of Thermo Fisher Scientific.

Behind the Bench

Multiplex vs. Singleplex qPCR: What do I need to know?

December 3, 2025

Read more Multiplex vs. Singleplex qPCR: What do I need to know?

From Bottlenecks to Breakthroughs: Scaling Molecular Testing with OpenArray

October 1, 2025

Read more From Bottlenecks to Breakthroughs: Scaling Molecular Testing with OpenArray

Trust your SYBR Green qPCR Data

November 3, 2022

Read more Trust your SYBR Green qPCR Data

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