Apoptotis cell image

Accurate apoptosis analysis in your choice assays

Many different approaches are used in the study of programmed cell death, or apoptosis. Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach for accurate assessment. Understanding the mechanisms of cell death and survival can represent a critical aspect of toxicological profiling and drug discovery.

We offer a wide array of apoptosis assays for measuring multiple components on a choice of assay platforms:

  • Caspase activity assays
  • DNA fragmentation and morphology
  • Annexin V staining
  • Membrane potential and other mitochondrial assays

Also see flow cytometry assays for apoptosis

Caspase Activity Assays   Caspase assays are a distinctive feature of early stage apoptosis.
  • Real-time no wash assay for caspase activity
  • Multi-parameter assays for live and dead cells
  • Antibodies for immunodetection of apoptosis
DNA Fragmentation and Morphology   DNA fragmentation and nuclear changes characterize late apoptosis.
  • TUNEL assays formatted for imaging and HCS
  • DNA stain in ready-to-use format
  • Compatible with multiplexed staining procedures
Annexin V Staining   Annexin V staining is used to identify intermediate apoptosis stages.
  • Alexa Fluor® dyes for more sensitive assays
  • Multiple conjugates for multiplexing flexibility
  • Simple protocol and rapid assays
Membrane Potential and Other Mitochondrial Assays   Mitochondrial changes occur early in apoptosis.
  • Real-time assays in live cells
  • Choice of physiological measurements
  • Fixable probes for multiplexing with immunodetection

Resources

Molecular Probes® Handbook
BioProbes® Journal Articles
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