Tools for flexible protocols, and fast, high-quality results

  • Speed—clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification
  • Precision—no extra sequences; clone what you want where you want
  • Flexibility—use our linearized vectors or any vector of your choice
  • Simplicityfree online tool  guides you through planning, design and assembly of your construct and PCR primer design, if needed

Expert Vector Construction: The GeneArt Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA. It’s what our experts use to make vectors, and they save 3-4 weeks versus traditional RE cloning methods.

Which Seamless Cloning & Assembly Kit is Right for You?

  Order now Order now Order now Order now Order now
  Assemble up to 4 fragments Assemble up to 4 fragments Assemble up to 4 fragments Assemble up to 8 fragments Assemble up to 10 fragments
  GeneArt Seamless Cloning Enzyme Mix GeneArt Seamless Cloning & Assembly Kit GeneArt Seamless PLUS Cloning and Assembly Kit GeneArt Type IIs Assembly kits GeneArt High-Order Genetic Assembly System
Cloning Efficiency >90%
  • 90% - single 5 kb DNA
  • 70% - 4 Fragments, 1 kb each
  • 40% - 4 DNA Fragments, 2 kb each
  • 90% - 5 fragments up to 2 kb each (direct)
  • > 90% - 8 fragments totaling 10 kb (pre-cloned)
All-in-one Enzyme/Buffer Mix    
# of Fragments Cloned Simultaneously Up to 4 Up to 4 Up to 4 Up to 8 Up to 10
Maximum Assembled Product Size Up to 13 kb (with vector) Up to 13 kb (with vector) Up to 40 kb (with vector)
  • Up to 13 kb (with vector)
  • Up to 20 kb to lower efficiency
Up to 110 kb (with vector)
Time to Clone Multiple Fragments Typically in half a day 1 day Typically in half a day Typically in half a day 3 days
Replication Host E. coli E. coli E. coli E. coli S. cerevisae MaV203
Assemble Fragments w/o End Sequence Homology w/o PCR      

GeneArt Web-Based Design Tool

Guides the assembly of fragments, checks for potential issues, designs DNA oligonucleotides and presents an annotated sequence and graphical representation of the final assembled molecule.

GeneArt Web-Based Design Tool


Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications

View Synthetic Biology webinars

Download the Seamless Cloning, Assembly, and Mutagenesis brochure

Compare Seamless Cloning to traditional cloning

Download the Type IIs Assembly flyer