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Pathology imaging with high-resolution transmission electron microscopy
Transmission electron microscopes (TEMs) provide crisp, high-contrast imaging that helps pathologists understand cell and tissue abnormalities at high resolution.
Accurate measurements of membranes, cellular organelles, and pathogens with TEM can be critical for understanding the tissue pathologies resulting from cell and organ dysfunctions, viral and microorganism infections, and cancer.
Precise measurements of the glomerular basement membrane in a kidney section. Data courtesy of the Department of Renal Pathology, Brigham and Women's Hospital, USA.
Talos 12 TEM for pathology imaging
The Thermo Scientific Talos 12 TEM provides reduced time to results through streamlined pathology workflows that can adapt to multi-user environments. Newer users can easily collect data while advanced users have access to more sophisticated controls.
Offline data viewing and collaboration, with integrated data annotation and storage, allows multiple team members to work together and analyze data simultaneously.
Talos 12 TEM – built for your pathology imaging needs.
Easy onboarding and improved time-to-results for pathology imaging
The complexity of legacy transmission electron microscopes can contribute to high operational and maintenance costs along with intensive onboarding for new users.
The Talos 12 TEM is equipped with Thermo Scientific Velox Software, a workflow-based user interface ideal for both novice and expert users, which helps reduce the TEM learning curve while increasing reproducibility and shortening time to results.
The Velox Software user interface enables easy onboarding for new users and improves time to results.
Velox Software supports pathology electron microscopy
Reduced learning curve
Velox Software offers a mode-dependent user interface with graphical layouts that make the software easier to learn and more intuitive.
High reproducibility
Combined comprehensive access to microscope optics and detectors provides superior experimental control for high reproducibility.
Improved time to results
Integrated auto functions ease the learning curve for novice operators and improve time-to-data for experienced users.
Effortlessly find and capture your regions of interest for pathology imaging
Fully integrated and embedded into the design of the Talos 12 TEM, Thermo Scientific Ceta CMOS Cameras provide high stability and optimized detector performance. These cameras offer the speed and high-contrast imaging necessary for seamless, live visualization during sample navigation. Velox Software also allows magnification to be adjusted once a region of interest is identified, helping to find and visualize structures of interest.
TEM image of resin-embedded muscle tissue at different magnifications. Velox Software enables easy magnification adjustments to capture regions of interest. Left) A larger ROI (cyan) and smaller ROI (yellow) are indicated within a TEM image. Center) A close up of the cyan ROI is shown with the yellow ROI within it. Right) A close up of the yellow ROI is shown.
Image rotation for convenient measurements
Velox Software enhances sample visualization by allowing you to rotate the image without removing the specimen from the microscope. It also enables automatic stage direction adjustments while keeping all raw data.
An overlay function can be used to create multiple distance measurements and annotations, which can be saved and exported for review or sharing.
Improved productivity with automated data collection
Thermo Scientific Maps 3 Software is an add-on for the Talos 12 TEM that facilitates automated data collection for large area imaging.
Navigate and scan large tissue section overviews, find regions of interest, and set-up automated multi-scale image acquisitions for unsupervised, high-resolution analysis of targeted areas. Increase productivity by multi-tasking during image collection or through the use of unattended and extended data-acquisition sessions.
Example electron microscopy workflow in renal pathology
TEM examination of a glomerular basement membrane with Maps 3 Software
Maps 3 Software improves productivity by streamlining automated data collection of large areas. In this example, the glomerular basement membrane in a kidney tissue sample is examined with the Talos TEM and Maps 3 Software. 1) An area to be imaged at high resolution is first identified. 2) A batch acquisition of tiles is then set up to encompass the target area (glomerulus). 3) Data is then automatically collected and stitched into seamless images. 4) Overview of the resulting stitched tile set. 5) Digital zoom into an area of interest. 6) Measurements and annotations can be added once data collection is completed. This can be done in collaboration with remote team members, and the data can be analyzed simultaneously. Image data courtesy of the Renal Pathology Department, Brigham and Women’s Hospital, USA.
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