ReadyProbes Cell Viability Imaging Kit (Blue/Red) Protocol
Two-color nuclear staining assay for cell viability
This kit can be used to quickly and easily determine the viability of cells. NucBlue Live reagent stains the nuclei of all cells, while propidium iodide stains only the nuclei of dead cells.
This protocol can be used for:
- Identifying live and dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
|1. Culture cells in appropriate medium and vessel for microscopy
|2. Add 2 drops each NucBlue Live and propidium iodide per milliliter of medium to label cells|
|3. Incubate 5–30 minutes
|4. Image cells
Spectral information and storage
|NucBlue Live||Propidium iodide|
|Excitation/Emission||360/460 nm||535/617 nm|
|Standard filter set||DAPI||TRITC or RFP|
|EVOS Light Cube||DAPI||TRITC or RFP|
|Storage conditions||Room temperature||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing media with Live Cell Imaging Solution
Jurkat cells stained with NucBlue Live reagent and propidium iodide.
For Research Use Only. Not for use in diagnostic procedures.