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Invitrogen
4-1BB (CD137/TNFRSF9) Mouse ELISA Kit
Product Specifications
Analytical sensitivity
6 pg/mL
Assay range
6.14-1500 pg/mL
Sample type/volume
Plasma
33 µL
Serum
33 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping condition
Wet or Dry Ice
Storage
2-8°C
Protein name
4-1BB (CD137)
Protein aliases
4-1BB ligand receptor, T-cell antigen 4-1BB, CD137, CD137 antigen, secreted CD137 antigen, Tumor necrosis factor receptor superfamily member 9
Protein family
Cytokines & Receptors
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
4-1BB, A930040I11Rik, AA408498, AI325004, Cd137, CDw137, ILA, Ly63, Tnfrsf9
Gene ID
Gene symbol
Tnfrsf9
UniProt ID
About This Kit
The Mouse 4-1BB (CD137/TNFRSF9) ELISA quantitates Ms 4-1BB in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms 4-1BB.
Principle of the method
The Mouse 4-1BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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