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Invitrogen
5-HIAA Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.94 ng/mL
Assay range
1.56-100 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
5-HIAA
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The 5 Hydroxyindoleacetic Acid (5 HIAA) ELISA quantitates 5 HIAA in serum, plasma and other biological fluids.
Principle of the method
The Competitive Gastrin (GT) ELISA research use only kit is designed to quantitatively measure GT in Humans. A GT standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human GT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
5-Hydroxyindoleacetic acid (5-HIAA) is the primary metabolite of serotonin (5-HT) and serves as a key marker for serotonin metabolism. As a more stable compound than serotonin, 5-HIAA is often measured in clinical settings to assess serotonin production and turnover. This measurement typically involves analyzing urine or plasma samples. Elevated levels of 5-HIAA in the urine can indicate various conditions such as carcinoid tumors, which are known to secrete large amounts of serotonin. Additionally, 5-HIAA has been implicated in inflammatory processes, notably acting as a potent ligand for the GPR35 receptor to promote neutrophil recruitment to inflamed tissues. The presence of 5-HIAA in cerebrospinal fluid is also used as a biomarker in neurological diseases impacting dopaminergic and serotoninergic pathways, although its levels can be influenced by age and other factors. The clinical relevance of 5-HIAA underscores its utility in diagnosing and monitoring various health conditions, particularly those involving serotonin dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 5-Hydroxyindoleacetic acid, urine
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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