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Invitrogen
In-Cell ELISA Kit, Near Infrared
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In-Cell ELISA Kit, Near Infrared
A431 cells were untreated or treated with 100 ng/mL EGF(green) or both 100 ng/mL EGF and 25 nM PD168393(purple)and measurements of pSTAT3, pSTAT6 and pEGFR were performed using the In-Cell ELISA Colorimetric Detection Kit. Results show incomplete inhibition of phosphorylation when cells are treated with PD168393.
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Product Specifications
Sample type/volume
Supernatant
10,000 cells
Hands-on time
1 hr 20 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Fluorescent Microplate Reader
Product size
4 x 96 Tests
Contents
Blocking Buffer
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Intracellular Proteins
Target site-specificity
Protein family
Other Proteins
Detector antibody conjugate
Unconjugated
Label or dye
DyLight™ 680 , DyLight™ 800
About This Kit
The In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells.
Principle of the method
This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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