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Invitrogen
Multispecies ERK1/ERK2 (Total/Phospho) InstantOne™ ELISA Kit
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FIGURE: 1 / 3
Multispecies ERK1/ERK2 (Total/Phospho) InstantOne™ ELISA Kit
Representative data for ERK1/ERK2 (Total/Phospho) Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor) for 3 hours (-) or treated with 10 ng/mL EGF for 10 min (+). Cells were analyzed for total (left) or phosphorylated (right) ERK 1/2 by InstantOne™ ELISA.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
ERK1/2
Target sub-specificity
Total
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human, mouse, and hamster ERK 1/2 in cell lysates. Detection of rat ERK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, HS44KDAP, HUMKER1A, MAPK1, MAPK2, MAPK3, NS13, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3
Gene ID : (Human) 5594, (Human) 5595
Gene symbol : MAPK1, MAPK3
Protein Aliases : ERK-1, ERK-2, erk1 erk2, ERK1b, p42, p42 MAP Kinase, Erk1, cancer susceptibility protein, ERK1c, Erk2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, p42-MAPK, p44-ERK1, p44-MAPK, protein tyrosine kinase ERK2, unnamed protein product
UniProt ID (Human) P28482, (Human) P27361
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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