Search
Invitrogen
Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
![Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/stat5-repeat-tds.jpg?time=20220826 "Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit")
FIGURE: 1 / 3
Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit
Representative data for STAT5 alpha/beta (Phospho) [pY694/pY699] Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band between 85 and 92 kDa as detected by immunoblot correlates wi... View More
![Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/stat5-repeat-tds.jpg?time=20220826)
![Human STAT5 alpha/beta (Phospho) [pY694/pY699] InstantOne™ ELISA Kit (85-86112-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20220826)
►
Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
STAT5 alpha/beta
Target site-specificity
Phosphorylated Tyr694, Phosphorylated Tyr699
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT5A/B in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Gene aliases : GHISID2, MGF, STAT5, STAT5A, STAT5B
Gene ID : (Human) 6776, (Human) 6777
Gene symbol : STAT5A, STAT5B
Protein Aliases : epididymis secretory sperm binding protein, Signal transducer and activator of transcription 5A, Signal transducer and activator of transcription 5B, STA5B, STAT, transcription factor STAT5B, unnamed protein product
UniProt ID (Human) P42229, (Human) P51692
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support