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Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit
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![Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/650/p-b-catenin-tds.jpg?time=20220826 "Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit")
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Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit
Representative data for beta Catenin (Phospho) [pS45] Multispecies InstantOne™ ELISA. A549 cells were either untreated (-) or treated with PMA/calyculin (+). Equal amounts of both untreated and treated cell lysates (5 µg) were analyzed at by either immunoblotting (left) or InstantOne™ ELISA (right). The intensity of the band at 94 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption va... View More
![Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit](https://www.thermofisher.com/antibody/images/150/p-b-catenin-tds.jpg?time=20220826)
![Multispecies beta Catenin (Phospho) [pS45] InstantOne™ ELISA Kit (85-86142-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20220826)
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
beta Catenin
Target site-specificity
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and rat beta-Catenin (Ser45) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Beta-catenin
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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