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Invitrogen
Multispecies GSK3 beta (Total/Phospho) InstantOne™ ELISA Kit
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FIGURE: 1 / 3
Multispecies GSK3 beta (Total/Phospho) InstantOne™ ELISA Kit
Representative data for GSK3 beta (Total/Phospho) Multispecies InstantOne™ ELISA. MCF7 cells were treated with either the PI3 Kinase inhibitor Wortmannin (10 µM) (-) or insulin (10 ng/mL for 10 min.) (+). Cell lysates were prepared as described in the InstantOne ELISA User Manual. Using the GSK3 beta (Total/Phospho) InstantOne ELISA, the cell lysates were analyzed for levels of Total GSK3 beta (left) and... View More
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
GSK3B
Target site-specificity
Target sub-specificity
Total
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : GSK-3 beta, Serine/threonine-protein kinase GSK3B
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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