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Invitrogen
Human LYVE-1 Uncoated ELISA Kit with plates
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Product Specifications
Analytical sensitivity
1.09 pg/mL
Assay range
1.09-70 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 15 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
2 x 96 Tests
Contents
Capture Antibody
Detection Antibody
Coating Buffer
Standard (2 vials)
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Chromogen
96 well microtiter plates (2)
Detection Antibody
Coating Buffer
Standard (2 vials)
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Chromogen
96 well microtiter plates (2)
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
LYVE1
Target site-specificity
Protein subtype
Adaptor Proteins
Protein family
Growth Factors & Receptors
Assay kit format
Uncoated ELISA Kit with Plates
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human LYVE-1 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human LYVE-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. A number of molecules have been identified as markers for lymphatic endothelium which include LYVE1, PALE, VEGFR3, and podoplanin. Diseases associated with LYVE1 dysfunction includes Complete Androgen Insensitivity Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Lymphatic vessel endothelial hyaluronic acid receptor 1, Cell surface retention sequence-binding protein 1, CRSBP-1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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