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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
This Human FGF2 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
FGF2
Protein family
Growth Factors & Receptors
Species (tested)
Human
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K08K consists of: 900-K08 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human FGF2 ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human FGF2 in a sandwich ELISA format. Human FGF2 ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : BFGF, FGF-2, FGFB, HBGF-2
Gene ID : (Human) 2247
Gene symbol : FGF2
Protein Aliases : 18 kd basic fibroblast growth factor, 21 kd basic fibroblast growth factor (ctg start codon, put.), putative, basic fibroblast growth factor, basic fibroblast growth factor (ctg start codon), basic fibroblast growth factor bFGF, FGF, fgf basic, FGF-b, Fibroblast growth factor, Fibroblast growth factor basic, H-FGF-b-147, H-FGF-b-154, M-FGF-b, FGF2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2, prostatropin
UniProt ID (Human) P09038
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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