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Invitrogen
Allopregnanolone ELISA Kit
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Product Specifications
Analytical sensitivity
129.7 pg/mL
Assay range
156.25-10,000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Fecal Extract
50 µL
Urine
12.5 µL
Supernatant
50 µL
Hands-on time
45 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
8%
Intraassay CV
7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Allopregnanolone
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Allopregnanolone Competitive ELISA quantitates allopregnanolone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure allopregnanolone independent of species. A allopregnanolone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit allopregnanolone antibodies and allopregnanolone-peroxidase conjugate is added to the wells. The allopregnanolone-peroxidase conjugate and any allopregnanolone in the sample will compete to bind to the anti-rabbit allopregnanolone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of allopregnanolone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 3alpha, 5alpha-tetrahydroprogesterone, 3alpha-hydroxy-5alpha-pregnan-20-one, allopregnanolone, Brexanolone, C21H34O2, Zulresso
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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