Invitrogen
Amyloid beta 42 Human ELISA Kit
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Analytical sensitivity
<10 pg/mL
Assay range
15.6-1000 pg/mL
Sample type/volume
Cerebrospinal Fluid
50 µL
Supernatants
50 µL
Tissue Homogenates
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
4.3%
Intraassay CV
3.6%
Instrument
Colorimetric Microplate Reader
Product size
96 tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping condition
Wet Ice
Storage
2-8°C
Protein name
Amyloid beta 42
UniProt ID
Q9UQ58,Q9UCD1,Q9UCC8,Q9UCB6,Q9UCA9,Q9UC33,Q9BT38,Q8WZ99,Q6GSC0,Q16020,Q16019,Q16014,Q16011,Q13793,Q13778,Q13764,P78438,P09000,D3DSD3,D3DSD2,D3DSD1,B4DII8,B2R5V1,P05067
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2
Gene ID
351
Gene symbol
APP
The Human Amyloid Beta 42 (Hu Abeta 42) ELISA quantitates Hu Abeta 42 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 42.
Principle of the method
The Human Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Beta-amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*
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