Invitrogen

Amyloid beta 42 Human ELISA Kit

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Product Details

Analytical sensitivity

<10 pg/mL

Assay range

15.6-1000 pg/mL

Sample type/volume

Cerebrospinal Fluid

50 µL

Supernatant

50 µL

Tissue Homogenate

50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr

Homogenous (no wash)

Interassay CV

4.3%

Intraassay CV

3.6%

Instrument

Colorimetric Microplate Reader

Product size

96 tests

Contents

Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping condition

Wet Ice

Storage

2-8°C

Protein name

Amyloid beta 42

Protein aliases

Abeta40, Abeta42, ABPP, AICD-50, AICD-57, AICD-59, Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, Cerebral vascular amyloid peptide, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), peptidase nexin-II, PN-II, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta, testicular tissue protein Li 2

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

Gene aliases

A4, AAA, ABETA, ABPP, AD1, APP, APPI, CTFgamma, CVAP, PN-II, PN2

Gene ID

(Human) 351

Gene symbol

APP

UniProt ID

(Human) P05067

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About This Kit

The Human Amyloid Beta 42 (Hu Abeta 42) ELISA quantitates Hu Abeta 42 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 42.

Principle of the method

The Human Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Beta amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.