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Invitrogen
Androstenedione Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
2.3 pg/mL
Assay range
5-1,200 pg/mL
Sample type/volume
Saliva
50 µL
Urine
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Androstenedione
Protein family
Chromone
Species (tested)
Chemical
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The multispecies Androstenedione ELISA quantitates androstenedione in serum, plasma, and saliva, or urine.
Principle of the method:
The Androstenedione Competitive ELISA research-use-only kit is designed to quantitatively measure androstenedione independent of species. An adrostenedione standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with donkey anti-sheep IgG antibodies. Rabbit polyclonal antibodies and androstenedione-peroxidase conjugate is added to the wells. The androstenedione conjugate and any androstenedione in the sample will compete to bind to the rabbit androstenedione antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound testosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of androstenedione present in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Androstenedione is a steroid hormone also known as androst-4-ene-3,17-dione. It is synthesized from dehydroepiandrosterone (DHEA) or 17-hydroxyprogesterone. By itself, androstenedione is a weak androgen though it is a precursor for biosynthesis of stronger androgens, such as testosterone and estrogens. Therefore, androstenedione is classified as a controlled drug and a supplement to enhance athletic performance and body energy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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