The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma, urine or cell culture medium.
Principle of the method
The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
ANP is derived from the 151 amino acid long protein preproANP3. The prepro protein is cleaved to generate proANP. ANP is derived from amino acids 99-126 to form the 28 amino acid peptide with a disulfide bond between amino acids 7 and 23. ANP is the predominant member of a family of structurally and functionally related peptide hormones that exert a wide array of effects on cardiovascular and renal function. ANP is identical in a large number of species. Human, pig, sheep, and dog ANP have identical sequences and this kit will measure samples from these species identically. Rat and mouse ANP molecules differ at amino acid 12 but show complete cross reactivity with this kit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.