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Invitrogen
Human CD40L (Soluble) Instant ELISA™ Kit
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Product Specifications
Analytical sensitivity
0.062 ng/mL
Assay range
0.16-10.0 ng/mL
Sample type/volume
Plasma
15 µL
Serum
15 µL
Supernatant
15 µL
Hands-on time
15 min
Time-to-result
2 hr 10 min
Homogenous (no wash)
No
Interassay CV
14.2%
Intraassay CV
6.8%
Instrument
Absorbance Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CD154 (CD40 Ligand)
Target sub-specificity
Soluble
Species (tested)
Human
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Soluble CD40 Ligand(Hu sCD40L ELISA quantitates Hu sCD40L in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sICD40L.
Principle of the method
The Human sCD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD154, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP
Gene ID : (Human) 959
Gene symbol : CD40LG
Protein Aliases : CD154, CD40 antigen ligand, CD40 ligand, CD40-L, H-CD-40-L, M-CD-40-L, RP23-153G22.3, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, TRAP, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
UniProt ID (Human) P29965
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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