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Invitrogen
Basic Human PD-L1 ELISA Kit
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Product Specifications
Analytical sensitivity
<0.6 pg/mL
Assay range
4.69-300 pg/mL
Sample type/volume
Serum
50 µL
Cell Lysate
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Biotinylated Detection antibody
Sample Diluent
Assay Buffer concentrate
Wash Buffer concentrate
SAV-HRP
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PD-L1 (CD274)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Kit Overview
The Human PD-L1 ELISA kit enables the quantification of Human PD-L1 in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PD-L1.
Principle of the assay
The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : B7-H, B7H1, CD274, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Gene ID : (Human) 29126
Gene symbol : CD274
Protein Aliases : B7 homolog 1, B7-H1, CD274, CD274 antigen, PD-L1, PDCD1 ligand 1, Programmed cell death 1 ligand 1, sCD274, soluble CD274, soluble PD L1, sPD L1, pdl-1
UniProt ID (Human) Q9NZQ7
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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