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Invitrogen
Basic Human PDGF-BB ELISA Kit
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Product Specifications
Analytical sensitivity
4.6 pg/mL
Assay range
31.3-2,000 pg/mL
Sample type/volume
Supernatant
20 µL
Serum
20 µL
Plasma
20 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PDGF-BB
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Kit Overview
The Human PDGF-BB ELISA kit enables the quantification of Human PDGF-BB in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PDGF-BB.
Principle of the assay
The Human PDGF-BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : c-sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV
Gene ID : (Human) 5155
Gene symbol : PDGFB
Protein Aliases : becaplermin, FLJ12858, H-PDGF-BB, PDGF subunit B, PDGF, B chain, PDGF-2, platelet-derived growth factor 2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), Platelet-derived growth factor subunit B, platelet-derived growth factor, beta polypeptide (oncogene SIS), Proto-oncogene c-Sis
UniProt ID (Human) P01127
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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