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Invitrogen
Basic Mouse IFN alpha ELISA Kit
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Product Specifications
Analytical sensitivity
7.48 pg/mL
Assay range
31.3-2,000 pg/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IFN alpha
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Kit Overview
The Mouse IFN alpha ELISA kit enables the quantification of Mouse IFN alpha in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Mouse IFN alpha.
Principle of the assay
The Mouse IFN alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Gm12597, Ifa1, Ifa2, Ifna1, Ifnaa, MuIFN-alpha-A, OTTMUSG00000007655
Gene ID : (Mouse) 15962, (Mouse) 242517, (Mouse) 15965
Gene symbol : Ifna1, Ifna15, Ifna2
Protein Aliases : ifa, IFN alpha 1/13, IFN alpha 1b, Interferon alpha1 (IFNA1), Interferon alpha-1/13 (IFN-alpha-1/13), Interferon alpha-D (IFN alphaD), MGC138207, MGC138505, MGC138507, IFN α, IFNAalphaIFN-alpha-1/13, IFNalpha, IFNα, Interferon alpha 13 (IFNA13), Interferon alpha leukocyte (IFL), IFN-alpha-1, IFN-alpha-2, interferon alpha 15, interferon alpha family gene 1, interferon alpha family, gene 2, Interferon alpha-1, interferon alpha-2, novel interferon alpha (Ifna) family member
UniProt ID (Mouse) P01572
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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