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Product Specifications
Analytical sensitivity
17.3 pg/mL
Assay range
100-3200 pg/mL
Sample type/volume
Fecal Extract
50 µL
Plasma
1 µL
Saliva
12.5 µL
Serum
1 µL
Supernatant
50 µL
Urine
6.25 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
8.1%
Intraassay CV
8.8%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping condition
Wet or Dry Ice
Storage
-20°C
Protein name
Cortisol
Protein aliases
C21H30O5
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Cortisol ELISA quantitates Cortisol in human serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.
Principle of the method
The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma, and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma, and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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