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Invitrogen
Epiandrosterone ELISA Kit
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Product Specifications
Analytical sensitivity
0.126 ng/mL
Assay range
0.098-100 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Fecal extract
50 µL
Urine
12.5 µL
Supernatant
50 µL
Hands-on time
45 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
16%
Intraassay CV
8%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Epiandrosterone
Species (tested)
Human
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Epiandrosterone Competitive ELISA quantitates epiandrosterone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Epiandrosterone Competitive ELISA research-use-only kit is designed to quantitatively measure epiandrosterone independent of species. An epiandrosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit epiandrosterone antibodies and epiandrosterone-peroxidase conjugate is added to the wells. The epiandrosterone-peroxidase conjugate and any epiandrosterone in the sample will compete to bind to the anti-rabbit epiandrosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound epiandrosterone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of epiandrosterone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Epiandrosterone is a dehydroepiandrosterone metabolite and a precursor of testosterone and estradiol with hypolipidemic and anabolic property. Epiandrosterone, a potential neurosteroid, appears to bind to the gamma-aminobutyric acid (GABA)/benzodiazepine-receptor complex (GABA-RC), acting as a negative non-competitive modulator of GABA-RC as well as signal through the N-methyl-D-aspartate receptor. In addition this agent inhibits the pentose phosphate pathway (PPP) thereby dilating blood vessels pre-contracted by partial depolarization. Also, epiandrosterone inhibits the synthesis of thromboxane A2 in activated platelets, reduces plasma plasminogen activator inhibitor type 1 and tissue plasminogen activator antigen, increases serum levels of insulin-like growth factor 1 and increases cyclic guanosine monophosphate and nitric oxide synthesis. These effects may improve circulation in the microvasculature. [National Center for Biotechnology Information. PubChem Compound Database; CID=441302].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 3beta-androsterone, 3Beta-hydroxy-5alpha-androstan-17-one, 5alpha-androstan-3beta-ol-17-one, C19H30O2, Epiandrosterone, Isoandrosterone, Trans-Androsterone
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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