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Invitrogen
Human Angiotensin 2 ELISA Kit
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Product Specifications
Analytical sensitivity
18.75 pg/mL
Assay range
31.25-2,000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Angiotensin II
Protein family
Serpin
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Angiotensin II (Ang-II) ELISA quantitates Ang-II in serum, plasma and other biological fluids
Principle of the method:
The Competitive Angiotensin II (Ang-II) ELISA research-use-only kit is designed to quantitatively measure Ang-II in Humans. An Ang-II standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human Ang-II is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human Ang-II in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AGT, ANHU, SERPINA8
Gene ID : (Human) 183
Gene symbol : AGT
Protein Aliases : alpha-1 antiproteinase, antitrypsin, angiotensin I, angiotensin II, Angiotensinogen, angiotensinogen (serpin peptidase inhibitor, clade A, member 8), FLJ92595, FLJ97926, pre-angiotensinogen, serine (or cysteine) proteinase inhibitor, Serpin A8, serpin peptidase inhibitor, member 8
UniProt ID (Human) P01019
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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