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Invitrogen
Human Basic IL-17A ELISA Kit
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Product Specifications
Analytical sensitivity
0.5 pg/mL
Assay range
1.6-100 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-17A
Protein family
Hormones & Receptors
Species (tested)
Human
Species (reported)
Human
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Interleukin-17A (Hu IL-17A) ELISA kit facilitates the accurate quantification of Hu IL-17A in various biological samples, including human serum, plasma, buffered solutions, or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of Hu IL-17A.
Principle of the method
The Human IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) utilizes a meticulously paired set of antibodies to capture and measure the target analyte. The microplate wells are pre-coated with a target-specific antibody, enabling the binding of the analyte during the assay. Following the addition of samples or standards to the wells, they bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
Target information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CTLA-8, CTLA8, IL-17, IL-17A, IL17, IL17A, ILA17
Gene ID : (Human) 3605
Gene symbol : IL17A
Protein Aliases : CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, IL-17, IL-17A, il 17, ILN, Interleukin, interleukin 17, Interleukin17A, R-IL-17-A, inflammatory cytokine, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin-17, Interleukin-17A
UniProt ID (Human) Q16552
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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