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Invitrogen
Human Big ET-1 ELISA Kit
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Product Specifications
Analytical sensitivity
0.185 pg/mL
Assay range
0.4-25 pg/mL
Sample type/volume
Cell Lysate
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
4.9%
Intraassay CV
4.7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Endothelin 1
Target site-specificity
Target sub-specificity
Soluble
Protein family
Vascular Protein
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Big ET-1 Platinum ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Big ET-1. Cell culture supernatant, serum, and plasma (EDTA, citrate, heparin) have been tested with this assay.
Target information
The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : EDN1, endothelin 1, Endothelin-1, ET1, HDLCQ7, PPET1, preproendothelin-1
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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