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Invitrogen
Human CD18 ELISA Kit
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Product Specifications
Analytical sensitivity
0.22 ng/mL
Assay range
0.22-50 ng/mL
Sample type/volume
Plasma
100 µL
Supernatant
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CD18
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human CD18 ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human Integrin beta 2/CD18 in serum, plasma, and cell culture supernatants.
Target information
CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD18, ITGB2, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7
Gene ID : (Human) 3689
Gene symbol : ITGB2
Protein Aliases : CD18, cell surface adhesion glycoprotein (LFA-1/CR3/P150, 959 beta subunit precursor), Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, complement component 3 receptor 3 and 4 subunit, complement receptor C3 beta-subunit, Complement receptor C3 subunit beta, integrin beta chain, beta 2, Integrin beta-2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
UniProt ID (Human) P05107
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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