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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
This Human CD154 (CD40 Ligand) ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.
Storage
Refer to product documentation for component specific storage temperature.
Protein name
CD154 (CD40 Ligand)
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
900-K145K consists of: 900-K145 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human CD154 (CD40 Ligand) ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human CD154 (CD40 Ligand) in a sandwich ELISA format. Human CD154 (CD40 Ligand) ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD154, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP
Gene ID : (Human) 959
Gene symbol : CD40LG
Protein Aliases : CD154, CD40 antigen ligand, CD40 ligand, CD40-L, H-CD-40-L, M-CD-40-L, RP23-153G22.3, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, TRAP, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5, unnamed protein product
UniProt ID (Human) P29965
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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