Invitrogen

Human CD99 ELISA Kit

Technical guide & protocol Tech Support

Product Details

Analytical sensitivity

0.062 ng/mL

Assay range

0.062-15 ng/mL

Sample type/volume

Serum

50 µL

Plasma

50 µL

Supernatant

50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping condition

Wet or Dry Ice

Storage

2-8°C

Protein name

CD99

Protein aliases

12E7, antigen identified by monoclonal 12E7, Y homolog, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99, CD99 antigen, cell surface antigen 12E7, cell surface antigen HBA-71, cell surface antigen O13, E2 antigen, MIC2 (monoclonal antibody 12E7), Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

Gene aliases

CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X

Gene ID

(Human) 4267

Gene symbol

CD99

UniProt ID

(Human) P14209

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About This Kit

Human CD99 quantitates human CD99 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD99.

Target information

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.