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Invitrogen
Human EphB4 ELISA Kit
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Product Specifications
Analytical sensitivity
16.3 pg/mL
Assay range
16.3-5,000 pg/mL
Sample type/volume
Supernatant
100 µL
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
EphB4
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human EphB4 ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human EphB4 in cell culture supernatants, plasma, and serum samples.
Target information
EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CMAVM2, EPHB4, HFASD, HTK, LMPHM7, MYK1, TYRO11
Gene ID : (Human) 2050
Gene symbol : EPHB4
Protein Aliases : ephrin receptor EphB4, Ephrin type-B receptor 4, Hepatoma transmembrane kinase, tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11, unnamed protein product
UniProt ID (Human) P54760
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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