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Invitrogen
Human FCGR3A/CD16A ELISA Kit
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Product Specifications
Analytical sensitivity
1.35 ng/mL
Assay range
1.311-320 ng/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
CD16
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human FCGR3A/CD16A quantitates human FCGR3A/CD16A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human FCGR3A/CD16A.
Target information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD16, CD16A, CD16B, FCG3, FCGR3, FCGR3A, FCGR3B, FCGRIII, FCR-10, FCRIII, FCRIIIA, FCRIIIb, IGFR3, IMD20
Gene ID : (Human) 2214, (Human) 2215
Gene symbol : FCGR3A, FCGR3B
Protein Aliases : CD16-I, CD16-II, CD16a, CD16a antigen, CD16b, CD32, CD32 receptor 2, CDw32, FCG2, Fc-gamma RII-b, Fc-gamma RII-c, FcRII-b, FcRII-c, RP11-5K23.1, Fc-gamma-RIIb, Fc-gamma-RIIc, FCGR2, FCGR2A, FCGR2B, FCGR2C, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG, low affinity III, receptor for (CD16), low affinity IIIa, receptor (CD16a), low affinity IIIb, receptor (CD16b), Fc gamma receptor III-A, Fc gamma receptor IIIa, Fc gamma receptor IIIb, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIII-beta, Fc-gamma RIIIa, fc-gamma RIIIb, FcgammaRIIIA, FcR-10, IgG Fc receptor III-1, IgG Fc receptor III-2, IgG Fc receptor III-A, immunoglobulin G Fc receptor III, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-B, neutrophil-specific antigen NA
UniProt ID (Human) P08637, (Human) O75015
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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