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Invitrogen
Human GAPDH ELISA Kit
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Product Specifications
Analytical sensitivity
0.94 ng/mL
Assay range
1.56-100 ng/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
GAPDH
Protein family
Glyceraldehyde-3-phosphate dehydrogenase
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human GAPDH ELISA quantitates GAPDH in serum, plasma, and other biological fluids
The Human GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CDABP0047, G3PD, GAPD, GAPD2, GAPDH, GAPDH-2, GAPDH2, GAPDHS, GAPDS, HEL-S-162eP, HEL-S-278, HSD-35, HSD35, OK/SW-cl.12
Gene ID : (Human) 2597, (Human) 26330
Gene symbol : GAPDH, GAPDHS
Protein Aliases : aging-associated gene 9 protein, EC 1.2.1.12, HGNC:4141, MGC88685, epididymis secretory protein Li 278, epididymis secretory sperm binding protein Li 162eP, GAPDH, GAPDH-2, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Peptidyl-cysteine S-nitrosylase GAPDH, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
UniProt ID (Human) P04406, (Human) O14556
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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