Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.25 ng/mL

Assay range

0.25-25 ng/mL

Sample type/volume

Supernatant
50 µL
Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

WFDC2

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human HE4/WFDC2 quantitates human HE4/WFDC2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human HE4/WFDC2.

Target information

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : dJ461P17.6, EDDM4, HE4, WAP5, WFDC2

Gene ID : (Human) 10406

Gene symbol : WFDC2

Protein Aliases : epididymal protein 4, Epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WAP domain containing protein HE4-V4, WAP four-disulfide core domain protein 2

UniProt ID (Human) Q14508

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