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Invitrogen
Human Haptoglobin Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.09 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Haptoglobin
Species (tested)
Human
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human HP (Haptoglobin) ELISA quantitates HP in serum and plasma.
Principle of the method
The Human Haptoglobin (HP) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Haptoglobin (HP) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Haptoglobin (HP). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Haptoglobin (HP) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Haptoglobin is an a2-globulin plasma protein synthesized in the liver. It is an acute phase protein which also acts as a scavenger of hemoglobin from hemolyzed red cells. The haptoglobin monomer consists of two a and two beta chains; the a chains bay be hp1Fa, hp1Sa, or hp2a types while the beta chain is constant. Three major phenotypes are recognized: HP1-1, Hp2-1 and Hp-2-2, in which the hp1a chain bay be either the F or S type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : HP2ALPHA2, HPA1S
Gene ID : (Human) 3240
Gene symbol : HP
Protein Aliases : alpha polypeptide, beta polypeptide, Binding peptide (BP), Haptoglobin alpha chain / alpha(1S) beta, Haptoglobin alpha(2FS) beta, HP2 ALPHA2, HP2-ALPHA-2, HPT, MGC111141, MGC133752, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin precursor, haptoglobin, preprohaptoglobin, unnamed protein product, zonulin
UniProt ID (Human) P00738
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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