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Invitrogen
Human ILT-2/LIR-1/CD85j ELISA Kit
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Product Specifications
Analytical sensitivity
22 pg/mL
Assay range
22-5,000 pg/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
CD85j (ILT2)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human ILT-2/LIR-1/CD85j quantitates human ILT-2/LIR-1/CD85j in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ILT-2/LIR-1/CD85j.
Target information
CD85j, also known as a member of the leukocyte immunoglobulin-like receptor (LIR) family, is a single transmembrane glycoprotein with a long cytoplasmic domain containing four immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs signal through interactions with SHP-1, transducing negative signals that inhibit immune response stimulation. CD85j is expressed on myeloid cells such as monocytes and dendritic cells, as well as on subsets of lymphoid cells including NK, T, and B cells. The expression of CD85j is correlated with leukemias such as acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). On CD8+ cells, CD85j expression is associated with effector cell function and plays a significant role in viral infections, including HIV, Epstein-Barr virus, and cytomegalovirus (CMV). CD85j binds to MHC class I molecules, including HLA-G, A, F, B27, E, and F, on antigen-presenting cells. The receptor helps control inflammatory responses and cytotoxicity, focusing the immune response and limiting autoreactivity. CD85j belongs to the subfamily B class of LIR receptors, which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and multiple transcript variants encoding different isoforms have been identified for this gene. Its gene is located in a cluster at chromosomal region 19q13.4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD85J, ILT-2, ILT2, LILRB1, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB
Gene ID : (Human) 10859
Gene symbol : LILRB1
Protein Aliases : CD85 antigen-like family member J, CD85j, Ig-like transcript 2, ILT-2, ILT2a, allelic form of ILT2, ILT2b, alternatively spliced form of ILT2, ILT2c, immunoglobulin heavy chain variable region, Immunoglobulin-like transcript 2, leucocyte Ig-like receptor B1, Leukocyte immunoglobulin-like receptor subfamily B member 1, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1, LIR-1, MIR-7, Monocyte/macrophage immunoglobulin-like receptor 7, myeloid inhibitory receptor 7, unnamed protein product
UniProt ID (Human) Q8NHL6
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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