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Invitrogen
Human IP-10/CXCL10 Instant ELISA™ Kit
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Product Specifications
Analytical sensitivity
1.0 pg/mL
Assay range
3.1-200 pg/mL
Sample type/volume
Amniotic Fluid
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Synovial Fluid
10 µL
Urine
10 µL
Hands-on time
15 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
10.4%
Intraassay CV
6.7%
Instrument
Absorbance Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CXCL10 (IP-10)
Species (tested)
Human
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu sIP-10/CXCL10 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, synovial fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIP-10/CXCL10.
Principle of the method
The Human sIP-10/CXCL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : C7, crg-2, CXCL10, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10
Gene ID : (Human) 3627
Gene symbol : CXCL10
Protein Aliases : 10 kDa interferon gamma-induced protein, Gamma-IP10, IP-10, Small-inducible cytokine B10, C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, CXC motif chemokine, CXCL, H-IP-10, M-IP-10, C-X-C motif chemokine 10, chemokine (C-X-C motif) ligand 10, gamma IP10, interferon-inducible cytokine IP-10, protein 10 from interferon (gamma)-induced cell line, small inducible cytokine subfamily B (Cys-X-Cys), member 10
UniProt ID (Human) P02778
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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