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Invitrogen
Human Integrin alpha-M/CD11b/MAC-1 ELISA Kit
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Product Specifications
Analytical sensitivity
2.5 ng/mL
Assay range
2.5-600 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
CD11b
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Integrin alpha-M/CD11b/MAC-1 quantitates human Integrin alpha-M/CD11b/MAC-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Integrin alpha-M/CD11b/MAC-1.
Target information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD11B, CR3A, ITGAM, MAC-1, MAC1A, MO1A, SLEB6
Gene ID : (Human) 3684
Gene symbol : ITGAM
Protein Aliases : antigen CD11b (p170), CD11 antigen-like family member B, CD11b, Cell surface glycoprotein MAC-1 subunit alpha, complement component 3 receptor 3 subunit, CR-3 alpha chain, glycoprotein Mac-1, Integrin alpha-M, integrin, alpha M (complement component 3 receptor 3 subunit), leukocyte adhesion glycoprotein precursor, Leukocyte adhesion receptor MO1, Mac-1 alpha (Mac1A), MGC117044, macrophage antigen alpha polypeptide, macrophage-1 antigen alpha subunit, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, unnamed protein product
UniProt ID (Human) Q4VAK1
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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