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Invitrogen
Human Marapsin/PRSS27 ELISA Kit
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Product Specifications
Analytical sensitivity
35 pg/mL
Assay range
35-10,000 pg/mL
Sample type/volume
Plasma
33 µL
Serum
33 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
PRSS27
Protein family
Cell Structure/Organelle Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Marapsin/PRSS27 ELISA quantitates Hu Marapsin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Marapsin.
Principle of the method
The Human Marapsin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
PRSS27, also known as Marapsin, is a gene located on chromosome 16p13.3 that encodes a serine protease enzyme. This protein is part of the trypsin-like serine protease family, which is characterized by a catalytic triad essential for proteolytic activity. PRSS27 is primarily expressed in the epidermis and other epithelial tissues, such as the salivary glands and pancreas. It plays a role in various physiological processes, including digestion, inflammation, and immune response regulation. In the skin, PRSS27 is involved in keratinocyte differentiation and the maintenance of skin barrier function. Dysregulation or altered expression of PRSS27 has been associated with inflammatory skin conditions, such as psoriasis, where it may influence the inflammatory cascade and contribute to disease pathogenesis. Research into PRSS27 continues to explore its potential role in epithelial homeostasis and as a therapeutic target for skin-related and other inflammatory disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CAPH2, MPN, PRSS27, UNQ1884/PRO4327
Gene ID : (Human) 83886
Gene symbol : PRSS27
Protein Aliases : channel-activating protease 2, Marapsin, Pancreasin, CAP-2, EC 3.4.21, EC 3.4.21.4, EC 3.4.21.43, MPNMarapsin, Serine protease 27
UniProt ID (Human) Q9BQR3
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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